MamoNaz

NAC the Nascent polypeptide Associated complex is a cytosolic protein complex serves as a gatekeeper in protein synthesis  which regulates the transport of protein within the cells. NAC is composed of NAC alpha and NAC beta .NAC binds reversibly to the ribosomes where it is in contact with nascent chains as they emerge from the ribosomes.   In Nascent polypeptide Associated complex is functionally versatile protein complex that is involved in protein biogenesis ,assembly and transportation and that plays a key role in maintaining protein homeostasis .In all eukaryotes NAC is composed of a heterodimer consisting of an alpha and beta subunits . The study shows the molecular function of NAC as gatekeeper, granting SRP only access for those nascent proteins whose destination is the endoplasmic reticulum.   Role of the Nascent polypeptide Associated complex:   NAC plays a key role in the attachment of cytosolic ribosomes to mitochondria and promotes translational distri...

  Insulin: Insulin is a natural hormone produced by your pancreas that controls how your body uses and stores blood sugar .It’s like a key that allows glucose to enter cells throughout your body. Insulin is a vital part of metabolism. Without it, your body would cease to function. When you eat, your pancreases releases insulin to help your body make energy out of glucose, a type of sugar found in carboxylates .It also helps you store energy. The use of insulin is associated with the increased risk of cardiovascular disease, cancer, kidney problem and all other mortality in comparison with other glucose-lowering therapies. The regular use of insulin: 1.    Sweating 2.    Dizziness 3.    Shakiness 4.    Hunger 5.    Fast heart rate 6.    Blurred vision 7.    Trouble confusion

  Forensic scientists contribute to legal debates by applying scientific methods to the examination of legal problems. Health care providers in this field include doctors, dentists and toxicologists. In many cases, they serve as frontline workers at the scene of a disaster or crime. Forensic nursing has changed how healthcare responds to specific patient populations, particularly in the setting relating to relationship violence, child maltreatment, elder care,   intentional  and unintentional  trauma investigation, death investigation ,corrections and, of course ,public health setting. From determining if a person is at an increased risk of developing certain genetic diseases and conditions, to understanding how the body metabolizes and reacts to certain medications, laboratory technicians can take a small sample of human tissue and discover extraordinary information that helps patients take charge of their health now and in years to come.

  DNA EXTRACTION: Principle: The extraction of DNA involve three major steps 1.        Cell lysis 2.        Protein separation 3.        DNA purification Procedure: 1. First add 1ml TE buffer into 200ul blood and mix it very well. 2. Centrifuge at 4000rpm 3. Discard the supernatant and add900 ul TE buffer. 4. Centrifuge at 4000rpm. 5. Discard the supernatant and add 800 ul TE buffer. 6. Centrifuge at 4000 rpm. 7. Discard the supernatant and add 200ul TEN buffer, 20ul SDS and 10ul proteinase-k. 8. Incubate at 56 degree. 9. Place the tubes on ice and add 6M nacl. 10. Centrifuge at 4000 rpm for 15 min. 11. Transfer the supernatant in a freshly labeled tubes. 12. Add equal amount of chilled isopropanol. 13. Centrifuge at 8000 rpm. 14. Add 200ul absolute ethanol 15. Centrifuge at 8000 rpm. 16. Add 200ul ethanol. 17. Centrifuge at 8000 rpm. 18. Discard the supernatant and add 100ul Low TE buffer. 19...